![]() Bacterial phyla that dominated the environment were Bacteroidetes, Acidobacteria, Planctomycetes, Actinobacteria and Verrucomicrobia. Alpha diversity indices suggested a high diversity and evenness of bacterial and fungal communities. ![]() Our analysis showed the presence of 25 elements and confirmed a high concentration of As and Tl. Trace metal content was detected using inductively coupled plasma optical emission spectrometry. We used a combination of high-throughput sequencing and bioinformatic analyses. The current study evaluated the bacterial and fungal diversity of three As- and Tl-contaminated sites in Allchar mineral mine. The mine is a well-known reservoir of the worldwide purest source of the thallium-bearing mineral, lorandite (TlAsS 2). The Allchar mineral mine is one of the oldest arsenic–antimony mines in the Republic of North Macedonia.
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